醫(yī)學(xué)診斷
0102030405
熱烈慶祝艾普拜生物科學(xué)家開(kāi)發(fā)最新的非侵入性腫瘤突變多重檢測(cè)方法（MPRP）發(fā)表于英國(guó)皇家化學(xué)學(xué)會(huì)雜志RSC Advances
2024-04-28
? ? ? ? ? 艾普拜生物（Apexbio）科學(xué)家于2018年8月在英國(guó)皇家化學(xué)學(xué)會(huì)雜志RSC Advances中發(fā)表了標(biāo)題為《非入侵性檢測(cè)腫瘤相關(guān)突變的滾環(huán)式多重real-time PCR方法（MPRP)》“Multiplex real-time PCR assay combined with rolling circle amplification (MPRP) using universal primers for non-invasive detection of tumor-related mutations”的文章。
? ? ? ? ? 文中所述的MPRP方法是將靈敏的多重real time PCR方法與滾環(huán)式DNA擴(kuò)增方法相結(jié)合，解決了很多其他基于PCR的SNP檢測(cè)方法中存在的靈敏度低、通量小、復(fù)雜的操作以及高成本等問(wèn)題，同時(shí)實(shí)現(xiàn)了便捷地多重real time PCR檢測(cè)。文中將MPRP方法與三通道微滴芯片式數(shù)字PCR平臺(tái)結(jié)果進(jìn)行比較，充分表明該方法在對(duì)靶向藥物的患者篩選和耐藥性預(yù)測(cè)中具備顯著的應(yīng)用前景。
用于多突變體檢測(cè)MPRP測(cè)定的示意圖 ↑
數(shù)字PCR測(cè)定的2D圖 ↑
紅圈中的點(diǎn)數(shù)表示陽(yáng)性微滴的數(shù)量
（A）患者P6的EGFR L858R測(cè)定的數(shù)字PCR結(jié)果
（B）患者P1的EGFR T790M測(cè)定的數(shù)字PCR結(jié)果
中文摘要：
? ? ? ? ? 隨著靶向藥物的持續(xù)開(kāi)發(fā)和應(yīng)用，需建立非入侵性診斷方法以便篩選可進(jìn)行治療的患者。而且多個(gè)腫瘤相關(guān)突變檢測(cè)對(duì)于治療和耐藥性預(yù)測(cè)意義重大。雖然有很多檢測(cè)SNP的PCR方法，但是由于靈敏度低、通量小、復(fù)雜的操作以及高成本等問(wèn)題，應(yīng)用嚴(yán)重受限。為解決上述問(wèn)題，本文描述的MPRP方法，將靈敏的多重real time PCR方法與滾環(huán)式DNA擴(kuò)增方法進(jìn)行了有效結(jié)合。提高了SNP檢測(cè)的特異性和靈敏度，同時(shí)實(shí)現(xiàn)便捷地多重real time PCR檢測(cè)。在8位患者樣本的MPRP方法與數(shù)字PCR方法的檢測(cè)結(jié)果比較中，充分表明MPRP方法具有潛在的臨床應(yīng)用前景。同時(shí)可以看出，MPRP方法是滿足腫瘤相關(guān)突變多重檢測(cè)的特異性和靈敏度要求且便捷的方法。
英文摘要：
With the continuous development and application of targeted drugs, it is particularly desirable to ?nd a non- invasive diagnostic approach to screen patients for precision treatment. Speci?cally, detection of multiple cancer-related mutations is very important for targeted therapy and prediction of drug resistance. Although numerous advanced PCR methods have been developed to discriminate single nucleotide polymorphisms, their drawbacks signi?cantly limit their application, such as low sensitivity and throughput, complicated operations, and expensive costs. In order to overcome these challenges, in this study, we? developed a method combining multiplex and sensitive real-time PCR assay with rolling circle ampli?cation. This allows speci?c and sensitive discrimination of the single nucleotide mutation and provides convenient multiplex detection by real-time PCR assay. The clinical potential of the MPRP assay was further demonstrated by comparing samples from 8 patients with a digital PCR assay. The coincident results between these two methods indicated that the MPRP assay can provide a speci?c, sensitive, and convenient method for multiplex detection of cancer-related mutations.
文獻(xiàn)原文請(qǐng)點(diǎn)擊如下鏈接：http://pubs.rsc.org/en/content/articlelanding/2018/ra/c8ra05259j#!divAbstract
